ABSTRACT
PURPOSE: Mesenchymal stem cells (MSCs) are surfacing as a new method of treatment for various diseases that have poor outcome with drug treatments. In this study, we investigated the effects of MSCs in a murine intestinal inflammation model mimicking human Crohn's disease (CD) using 2,4,5-trinitrobenzene sulfonic acid (TNBS). METHODS: Colitis was induced by rectal administration of 2 mg of TNBS in 35% ethanol as experimental group compared to control group. Histological changes, surface molecules of T and B cells of the spleen and blood, and cytokine production (IFN-gamma, TNF-alpha, IL-4, IL-6, IL-10, and IL-12) were determined among 3 groups comprised of control group, TNBS group and TNBS/MSC group. RESULTS: In the mice treated with MSCs, there was a decrease in the wasting disease process and inflammatory histopathological changes. There was also a decrease in pro-inflammatory T-helper 1 (Th1) cytokines IFN-gamma and IL-12 and T-helper 2 (Th2) cytokine IL-4. Anti-inflammatory cytokine IL-10 increased in mice treated with MSCs compared to colitic mice. The blood CD4+CD25+ T-regulatory cells also increased and splenic CD19 B-cells decreased. CONCLUSION: The results of this study suggest that MSCs may have a therapeutic effect in controlling the Th1 and Th2 mediated immune response in patients with CD and aid in tissue regeneration.
Subject(s)
Animals , Humans , Mice , Administration, Rectal , B-Lymphocytes , Colitis , Crohn Disease , Cytokines , Ethanol , Inflammation , Inflammatory Bowel Diseases , Interleukin-10 , Interleukin-12 , Interleukin-4 , Interleukin-6 , Mesenchymal Stem Cells , Regeneration , Spleen , Tumor Necrosis Factor-alpha , Wasting SyndromeABSTRACT
PURPOSE: Kawasaki Disease(KD) is a febrile disease with acute multisystemic vasculitis associated with early development of acute myocarditis and coronary artery abnormalities. The cause and etiologic agents are still unknown. Clinical studies suggest that KD is one of the autoimmune disorders caused by infectious agent, but this is not proved yet. The study was performed to investigate the levels of vascular endothelial growth factor(VEGF) in the acute stage of KD before treatment of intravenous-gammaglobulin(IVGG) to confirm and find an early diagnostic method of vasculitis by serologic test. METHODS: The patient group consisted of 61 cases in acute stages of KD before treatment of IVGG who were hospitalized from Jan. 1998 to Feb. 2001. The control group consisted of 24 cases among the admitted cases who suffered from non KD. The levels of VEGF in the obtained sera were measured by using chemoluminoimmunoassay, and those of both groups were compared to each other. RESULTS: The sex ratio of male to female was 1.6:1, males were more prone to infection, and mean age was 2.6+/-1.6 years old. The levels of VEGF in patients group(143.64+/-115.38 pg/mL) were significantly increased as compared with control group(26.55+/-13.75 pg/mL)(P<0.05). One case of acute myocarditis and two cases of left coronary artery dilations occurred in the patient group, but they all recovered after treatment two months later. Acute phage reactants such as CRP, ESR were positive and WBC was increased in acute stages of both groups. CONCLUSION: The significant increasing level of serum VEGF in acute febrile stages of KD suggests that acute vasculitis began in the early stage in all of the patients group, and the measurement of VEGF can be one of useful method for early diagnosis of vasculitis in KD. This may be helpful in early IVGG treatment of the condition.
Subject(s)
Female , Humans , Male , Bacteriophages , Coronary Vessels , Early Diagnosis , Mucocutaneous Lymph Node Syndrome , Myocarditis , Serologic Tests , Sex Ratio , Vascular Endothelial Growth Factor A , VasculitisABSTRACT
PURPOSE: The study was performed to investigate the IgG subclasses in acute febrile phase of Kawasaki disease(KD) to find correlation between selective IgG4 deficiency and incidence of KD patients. METHODS: The patients group consisted of 64 cases in acute phase of KD hospitalized from Jan. 1996 to Jul. 1999, and control group consisted of age matched 36 cases who admitted without KD. The obtained sera were measured the level of total IgG, IgM, IgA and IgG subclasses(IgG1, IgG2, IgG3, IgG4) by using Enzyme Immuno Assay and Single Radial Immuno-Diffusion method. RESULTS: The sex ratio of male to female was 1.4:1.0, and febrile days before admission was 4.6+/-2.4 days. Total levels of IgG, IgM and IgA were normal range in both groups. The levels of IgG1, IgG2 and IgG3 were not specific changes but significantly decreased the level of IgG4 in patients group as compared with control group(P<0.05). CONCLUSION: Selective decreased the levels of serum IgG4 can be one of factors to increasing the incidence of KD.
Subject(s)
Female , Humans , Male , Immunoglobulin A , Immunoglobulin G , Immunoglobulin M , Incidence , Mucocutaneous Lymph Node Syndrome , Reference Values , Sex RatioABSTRACT
No abstract available.